H-DBAS - Human-transcriptome DataBase for Alternative Splicing

	RELEASE 4.0 (Jan. 30, 2009) Transcripts from H-InvDB 6.0 (DDBJ 73 in origin) Genomes from UCSC hg18 (human) and mm9 (mouse)
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Welcome to H-DBAS - Human-transcriptome DataBase for Alternative Splicing

H-DBAS offers unique data and viewer for human Alternative Splicing (AS) analysis

Genome-wide representative alternative splicing variants (RASVs) identified from following datasets
- H-Inv full-length cDNAs (resource summary): H-Invitational cDNA dataset
- H-Inv all transcripts (resource summary): Published human mRNA dataset
- Mouse full-length cDNAs (resource summary): Mouse cDNA dataset
RASVs affecting protein functions such as protein motif, GO, subcellular localization signal and transmembrane domain
Conserved RASVs compared with mouse genome and the full-length cDNAs (H-Inv full-length cDNAs only)
AS Viewer (sample) as user-controllable Java applet and the followings are product requirments
- Java: JRE 1.5.0_17 (Free Java Download)
- OS: WindowsXP/Vista or Mac10.4 (Windows is recommended)
- Browser: IE6/7 (Windows) or Safari (Mac)
- Screen resolution: 1280 x 1024

Advantages of H-DBAS

Not using transcripts predicted by ESTs
Removing truncated transcripts determined by the result of mapping and clustering
Providing only non-redundant AS variants, i.e. RASVs
Using full-length cDNAs cloned by validated methods like Oligo-capping (H-Inv full-length cDNAs only)
Providing also biologically important RASVs conserved between human and mouse (H-Inv full-length cDNAs only)

Site flow

References

Takeda, J. et al. (2010) H-DBAS: human-transcriptome database for alternative splicing: update 2010. Nucleic Acids Research 38 (Database Issue), D86-D90 New!
Takeda, J. et al. (2008) Low conservation and species-specific evolution of alternative splicing in humans and mice: comparative genomics analysis using well-annotated full-length cDNAs. Nucleic Acids Research 36 (20), 6386-6395
Takeda, J. et al. (2007) H-DBAS: Alternative splicing database of completely sequenced and manually annotated full-length cDNAs based on H-Invitational. Nucleic Acids Research 35 (Database Issue), D104-D109
Takeda, J. et al. (2006) Large-scale identification and characterization of alternative splicing variants of human gene transcripts using 56,419 completely sequenced and manually annotated full-length cDNAs. Nucleic Acids Research 34 (14), 3917-3928
Imanishi,T. et al. (2004) Integrative annotation of 21,037 human genes varidated by full-length cDNA clones. PLoS Biology 2 (6), 856-875

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